FURTHER CHARACTERIZATION OF THE TS453 MUTANT OF MAMMALIAN ORTHOREOVIRUS SEROTYPE-3 AND NUCLEOTIDE-SEQUENCE OF THE MUTATED S4 GENE

被引：21

作者：

DANIS, C ^{[1
]}

GARZON, S ^{[1
]}

LEMAY, G ^{[1
]}

机构：

[1] UNIV MONTREAL,DEPT MICROBIOL & IMMUNOL,POB 6128,STN A,MONTREAL H3C 3J7,QUEBEC,CANADA

来源：

VIROLOGY | 1992年 / 190卷 / 01期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0042-6822(92)91241-L

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The sigma 3 protein of mammalian orthoreoviruses has multiple proven and postulated roles during viral multiplication. In this manuscript we took advantage of the availability of the ts453 thermosensitive mutant, already assigned to the S4 gene encoding sigma 3, to begin the elucidation of the relationship between the two main domains and the different roles of the sigma 3 viral protein. The alteration in the mutant appeared to affect the structural role of the protein. Nucleotide sequence determination indicated an especially significant change close to the zinc finger of the protein. These data suggest that the zinc-binding region might be especially important during the assembly of sigma 3 into the viral capsid. © 1992.

引用

页码：494 / 498

页数：5

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