IMMUNOGLOBULIN-CHI LIGHT CHAIN GERM-LINE TRANSCRIPTS IN HUMAN PRECURSOR LYMPHOCYTES-B

被引:22
作者
THOMPSON, A [1 ]
TIMMERS, E [1 ]
KENTER, MJH [1 ]
KRAAKMAN, MEM [1 ]
HENDRIKS, RW [1 ]
SCHUURMAN, RKB [1 ]
机构
[1] UNIV HOSP LEIDEN,DEPT IMMUNOHAEMATOL,DIV IMMUNOBIOL & GENET,BLDG 1E3-Q,POB 9600,2300 RC LEIDEN,NETHERLANDS
关键词
D O I
10.1002/eji.1830221221
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
B lymphoblastoid cell lines (BLCL), established from bone marrow and peripheral blood mononuclear cells from two severe combined immunodeficiency (SCID) patients, manifested a complete absence of genomic rearrangements of the immunoglobulin (Ig) heavy (H) and light (L) chain loci. The BLCL contained germ-line transcripts of the Ig kappa region locus of approximately 1.2 kilobases (kb). By cDNA cloning and sequence analysis the transcripts were shown to consist of a C(kappa) segment, a J(kappa)1 gene segment, 160 base pairs (bp) of J(kappa)1 5' intervening sequence, containing the heptamer/nonamer recombination recognition sequences and at the 5' end a 523-bp segment designated human kappa-degrees, The first 206 bp of this 5' segment were homologous to the reported murine kappa-degrees region. Genomic restriction mapping and DNA sequence analysis demonstrated that the human kappa-degrees segment is located approximately 4 kb upstream of J.1. The kappa-degrees segment contains a putative promoter region with an OCT2 binding site, and has a splice donor site to accomplish splicing to an acceptor site 160 bp upstream of J(kappa)1. Expression of the kappa-degrees gene segment was found in BLCL derived from normal fetal bone marrow, in which both Ig kappa loci were in the germ-line configuration. These findings indicate that the described transcripts are not only present in SCID, but also in normal developing pre-B lymphocytes. The expression of germ-line Ig kappa L chain transcripts may be associated with the locus becoming accessible to gene rearrangement.
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页码:3167 / 3171
页数:5
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