CLONING AND EXPRESSION OF A CDNA FOR THE RAT KAPPA-OPIOID RECEPTOR

被引：221

作者：

MINAMI, M

TOYA, T

KATAO, Y

MAEKAWA, K

NAKAMURA, S

ONOGI, T

KANEKO, S

SATOH, M

机构：

[1] KYOTO UNIV,FAC PHARMACEUT SCI,DEPT MOLEC PHARMACOL,KYOTO 60601,JAPAN

[2] KYOTO UNIV,FAC PHARMACEUT SCI,DEPT PHARMACOL,KYOTO 60601,JAPAN

来源：

FEBS LETTERS | 1993年 / 329卷 / 03期

关键词：

OPIOID RECEPTOR; KAPPA-TYPE; DYNORPHIN-A; U50488H;

D O I：

10.1016/0014-5793(93)80240-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We cloned a cDNA for the rat kappa-opioid receptor from a rat thalamus cDNA library. The deduced amino acid sequence consists of 380 residues with features shared by members of the G protein-coupled receptor family. The specific binding of [H-3]bremazocine to the membrane of COS-7 cells transfected with the cDNA was displaced by kappa-specific opioid ligands, but not by mu- and delta-specific ligands. Xenopus oocytes injected with the in vitro transcribed mRNA responded to opioid ligands with the same subtype specificity. Northern blot analysis demonstrated that kappa-opioid receptor mRNA is expressed in a regionally specific manner in rat brain.

引用

页码：291 / 295

页数：5

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