A NEW SYSTEM FOR THE EXPRESSION OF RECOMBINANT ANTIBODY IN MAMMALIAN-CELLS

被引：5

作者：

IBA, Y

KANEKO, T

EKIDA, T

MIYATA, K

INOUYE, K

KUROSAWA, Y

YASUKAWA, K

机构：

[1] TOSOH CORP,TOKYO RES CTR,AYASE,KANAGAWA 252,JAPAN

[2] KYOTO UNIV,FAC AGR,DEPT FOOD SCI & TECHNOL,SAKYO KU,KYOTO 60601,JAPAN

[3] FUJITA HLTH UNIV,INST COMPREHENS MED SCI,TOYOAKE,AICHI 47011,JAPAN

来源：

BIOTECHNOLOGY LETTERS | 1995年 / 17卷 / 02期

关键词：

D O I：

10.1007/BF00127976

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

New type of plasmids named pEdHCG1 and pEdHC kappa.were constructed. A combination of two plasmids containing the amplified gene product for the variable region of the antibody (Ab) heavy or light chains by the polymerase chain reaction (PCR) was co-transfected into COS cells to transiently express and in Chinese hamster ovary (CHO) cells to constitutively express chimeric Ab composed of the mouse-derived variable region and human-derived constant region.

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收藏

页码：135 / 138

页数：4

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