GAMMA-CARBOXYGLUTAMATE ANALYSIS BY SELECTIVE ANION-EXCHANGE ELUTION AND FLUORESCENT DETECTION

被引：30

作者：

GUNDBERG, CM

LIAN, JB

GALLOP, PM

机构：

[1] Department of Biological Chemistry and Oral Biology, Harvard Medical School, Harvard School of Dental Medicine, and Orthopaedic Research, Boston, MA 02115

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 98卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90730-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, sensitive method for the determination of free γ-carboxyglutamic acid (γ-CG) in urine and in the alkaline hydrolysates of proteins is presented. An aliquot of urine or protein hydrolysate containing added γ-[14C]CG is chromatographed on Dowex 1 employing stepwise treatments in N-(2-hydroxyethyl)piperazine-N′-2-ethanesulfonic acid buffers and final selective elution of γ-CG with MgCl2. Aliquots of the eluted γ-CG fractions are counted to determine percentage recovery and assayed for γ-CG content by fluorescence employing o-phthalaldehyde. This procedure correlates well with direct determination of γ-CG by the established procedure of automatic amino acid analysis. © 1979.

引用

页码：219 / 225

页数：7

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