YEAST CASEIN KINASE-I HOMOLOGS - AN ESSENTIAL GENE PAIR

被引:124
作者
ROBINSON, LC
HUBBARD, EJA
GRAVES, PR
DEPAOLIROACH, AA
ROACH, PJ
KUNG, C
HAAS, DW
HAGEDORN, CH
GOEBL, M
CULBERTSON, MR
CARLSON, M
机构
[1] COLUMBIA UNIV, DEPT GENET & DEV, NEW YORK, NY 10032 USA
[2] INDIANA UNIV, SCH MED, DEPT BIOCHEM & MOLEC BIOL, INDIANAPOLIS, IN 46223 USA
[3] VANDERBILT UNIV, MED CTR, SCH MED, DEPT MED, NASHVILLE, TN 37232 USA
[4] VANDERBILT UNIV, MED CTR, SCH MED, DEPT CELL BIOL, NASHVILLE, TN 37232 USA
[5] VET ADM MED CTR, NASHVILLE, TN 37232 USA
关键词
SACCHAROMYCES-CEREVISIAE; PROTEIN KINASE; SNF MUTATIONS; SALT TOLERANCE;
D O I
10.1073/pnas.89.1.28
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
We report the isolation of an essential pair of Saccharomyces cerevisiae genes that encode protein kinase homologues. The two genes were independently isolated as dosage-dependent suppressors. Increased dosage of YCK1 suppressed defects caused by reduced SNF1 protein kinase activity, and increased dosage of YCK2 relieved sensitivity of wild-type cells to salt stress. The two genes function identically in the two growth assays, and loss of function of either gene alone has no discernible effect on growth. However, loss of function of both genes results in inviability. The two predicted protein products share 77% overall amino acid identity and contain sequence elements conserved among protein kinases. Partial sequence obtained for rabbit casein kinase I shares 64% identity with the two yeast gene products. Moreover, an increase in casein kinase I activity is observed in extracts from cells overexpressing YCK2. Thus YCK1 and YCK2 appear to encode casein kinase I homologues.
引用
收藏
页码:28 / 32
页数:5
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