EFFECT OF DILUTION, INCUBATION-TIME, AND TEMPERATURE OF ENRICHMENT ON CULTURAL AND PCR DETECTION OF VIBRIO-CHOLERAE OBTAINED FROM THE OYSTER CRASSOSTREA-VIRGINICA

被引：15

作者：

DEPAOLA, A ^{[1
]}

HWANG, GC ^{[1
]}

机构：

[1] NATL FISHERIES RES & DEV AGCY,YANGSAN GUN 626900,SOUTH KOREA

来源：

MOLECULAR AND CELLULAR PROBES | 1995年 / 9卷 / 02期

关键词：

VIBRIO CHOLERAE; RECOVERY; OYSTER; CRASSOSTREA VIRGINICA; POLYMERASE CHAIN REACTION;

D O I：

10.1016/S0890-8508(95)80031-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The recovery of Vibrio cholerae O1 by culture from the oyster Crassostrea virginica and detection of the cholera toxin gene by polymerase chain reaction were evaluated using various enrichment procedures in alkaline peptone water. The effects of dilutions (1:10 and 1:100), incubation times (6-8 and 18-21 h), and incubation-temperatures (35 and 42 degrees C) were determined. Recovery of V. cholerae was significantly greater (P<0.05) from oyster homogenates diluted 1:100 in alkaline peptone water and incubated at 42 degrees C for 18-21 h. This enrichment procedure also provided the best detection of the cholera toxin gene by polymerase chain reaction.

引用

页码：75 / 81

页数：7

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