ONTOGENETIC DEVELOPMENT OF ANTIBODY FORMATION IN RESPONSE TO DIFFERENT DOSES OF GRAM-NEGATIVE MICROORGANISMS IN YOUNG RABBITS .2. RELATIONSHIP BETWEEN SECONDARY REACTION AND PRIMARY ANTIGEN DOSE AND AN ATTEMPT TO INDUCE TOLERANCE

Newborn and 15-day-old rabbits were immunized with different doses of heat-inactivated suspension of Escherichia coli and Salmonella paralyphi B. The secondary immunization was performed after 4 weeks and the dynamics, magnitude and site of the secondary response studied. The magnitude of the secondary response was found to depend on the magnitude and rate of the primary response, this latter reflecting the dose used. A direct relationship was found in the range of the minimal and optimal dose: the higher the primary dose the higher the secondary response. After a rapid and pronounced primary response evoked by a high dose of 1010 microorganisms in 15-day-old rabbits, a partial inhibition of the secondary response was observed. The inhibition was pronounced primarily in the spleen; the lymphatic nodes reacted by a higher number of antibody producing cells as compared with the control nonprimed young rabbits. © 1969 Academia, nakladatelství Československé akademie věd.