PEPTIDE SEQUENCING WITH ELECTROSPRAY IONIZATION ON A MAGNETIC-SECTOR MASS-SPECTROMETER

被引：49

作者：

MENG, CK ^{[1
]}

MCEWEN, CN ^{[1
]}

LARSEN, BS ^{[1
]}

机构：

[1] DUPONT CO,DEPT CENT RES & DEV,EXPTL STN,WILMINGTON,DE 19880

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 1990年 / 4卷 / 05期

关键词：

D O I：

10.1002/rcm.1290040505

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Sequence information is obtained by in‐source fragmentation of electrospray‐generated peptide ions. The complete sequences of leucine enkephalin, angiotensin I, and renin substrate are determined from their fragment ions and consume only low picomole amounts of material. The method involves collision‐induced fragmentation of singly and multiply charged molecular ions in the electrospray ion source and does not involve tandem mass spectrometric instrumentation. The charge on the fragment ions is determined by the spacing of the isotope peaks. Copyright © 1990 Heyden & Son Limited

引用

页码：151 / 155

页数：5

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