PREPARATION OF GULURONATE LYASE FROM PSEUDOMONAS-ALGINOVORA FOR PROTOPLAST ISOLATION IN LAMINARIA

被引：44

作者：

BOYEN, C ^{[1
]}

BERTHEAU, Y ^{[1
]}

BARBEYRON, T ^{[1
]}

KLOAREG, B ^{[1
]}

机构：

[1] INRA, PATHOL VEGETALE LAB, F-75231 PARIS 05, FRANCE

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1990年 / 12卷 / 11期

关键词：

Alginate-lyase; Laminaria sp; protoplast isolation; Pseudomonas alginovora;

D O I：

10.1016/0141-0229(90)90027-N

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A marine aerobic bacterium identified as Pseudomonas alginovora was isolated from decaying fronds of Laminaria digitata. It secreted an endo-acting alginate lyase of the guluronate-specific type. The enzyme was purified to homogeneity in SDS-PAGE by precipitation with ammonium sulphate, gel filtration, and anion-exchange chromatography. The purified fractions were effective in producing protoplasts from Laminaria species. © 1990.

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页码：885 / 890

页数：6

相关论文

共 30 条

[1] TAXONOMY OF AEROBIC MARINE EUBACTERIA [J].

BAUMANN, L ;

BAUMANN, P ;

ALLEN, RD ;

MANDEL, M .

JOURNAL OF BACTERIOLOGY, 1972, 110 (01) :402-+

[2] DETECTION OF DEPOLYMERASE ISOENZYMES AFTER ELECTROPHORESIS OR ELECTROFOCUSING, OR IN TITRATION CURVES [J].

BERTHEAU, Y ;

MADGIDIHERVAN, E ;

KOTOUJANSKY, A ;

NGUYENTHE, C ;

ANDRO, T ;

COLENO, A .

ANALYTICAL BIOCHEMISTRY, 1984, 139 (02) :383-389

[3] NEW METHOD FOR QUANTITATIVE-DETERMINATION OF URONIC ACIDS [J].

BLUMENKR.N ;

ASBOEHAN.G .

ANALYTICAL BIOCHEMISTRY, 1973, 54 (02) :484-489

[4] STRUCTURAL STUDIES OF ALGINIC ACID, USING A BACTERIAL POLY-ALPHA-L-GULURONATE LYASE [J].

BOYD, J ;

TURVEY, JR .

CARBOHYDRATE RESEARCH, 1978, 66 (OCT) :187-194

[5] PREPARATION OF ALGINATE LYASES FROM MARINE MOLLUSKS FOR PROTOPLAST ISOLATION IN BROWN-ALGAE [J].

BOYEN, C ;

KLOAREG, B ;

POLNEFULLER, M ;

GIBOR, A .

PHYCOLOGIA, 1990, 29 (02) :173-181

[6] ISOLATION CONDITIONS FOR HIGH YIELDS OF PROTOPLASTS FROM LAMINARIA-SACCHARINA AND LAMINARIA-DIGITATA (PHAEOPHYCEAE) [J].

BUTLER, DM ;

OSTGAARD, K ;

BOYEN, C ;

EVANS, LV ;

JENSEN, A ;

KLOAREG, B .

JOURNAL OF EXPERIMENTAL BOTANY, 1989, 40 (220) :1237-1246

[7] PROPERTIES OF ALGINATE LYASES FROM MARINE-BACTERIA [J].

DOUBET, RS ;

QUATRANO, RS .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1984, 47 (04) :699-703

[8] STUDY OF THE REACTION CATALYZED BY ALGINATE LYASE-VI FROM THE SEA MOLLUSK, LITTORINA SP [J].

FAVOROV, VV ;

VOZHOVA, EI ;

DENISENKO, VA ;

ELYAKOVA, LA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 569 (02) :259-266

[9] ALGINATES [J].

GACESA, P .

CARBOHYDRATE POLYMERS, 1988, 8 (03) :161-182

[10]

GILSON DM, 1977, IDENTIFICAITON SOME, P135