COMPARISON OF THE FLUORESCENT-ANTIBODY METHOD AND A STANDARDIZED CULTURAL METHOD FOR THE DETECTION OF SALMONELLAS

The results of routine use of the indirect fluorescent antibody (FA) technique using the Spicer‐Edwards H antisera set are reported for a range of agricultural and food samples. The FA technique was used on samples after the pre‐enrichment incubation period in the proposed ISO method for isolation of salmonellas. The numbers of FA false positive samples (ca. 5% overall) and FA false negative samples (ca. 1·3%) were low, but some originally FA false positive results were later shown to be false negative cultural results. Copyright © 1979, Wiley Blackwell. All rights reserved