A CONSTITUTIVE 65-KDA CHONDROCYTE PROTEIN AS A TARGET ANTIGEN IN ADJUVANT ARTHRITIS IN LEWIS RATS

被引：9

作者：

FEIGE, U

SCHULMEISTER, A

MOLLENHAUER, J

BRUNE, K

BANG, H

机构：

[1] Institute of Experimental and Cinical Pharmacology and Toxicology, University of Erlangen-Nurnberg, D-8250, Erlangen

[2] Rush Medical School, Department of Biochemistry, Chicago, IL, 60612

来源：

AUTOIMMUNITY | 1994年 / 17卷 / 03期

关键词：

ADJUVANT ARTHRITIS; AUTOANTIGEN; CYTOKERATIN; HEAT-SHOCK PROTEIN; AUTOIMMUNE DISEASE; IMMUNOTHERAPY;

D O I：

10.3109/08916939409010659

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The autoantigen in adjuvant arthritis in Lewis rats is still unknown despite the knowledge that the 65 kDa mycobacterial heat-shock protein (hsp) is involved in the disease process. T cells and antibodies obtained from rats with adjuvant arthritis respond to chondrocyte membrane antigen(s). In Western blots a 65 kDa chondrocyte membrane protein (CH65) is stained by sera from arthritic rats. In addition, spleen cells from rats with adjuvant arthritis proliferate in vitro to chondrocyte membranes and CH65 as antigens. Furthermore, pretreatment of rats with CH65 or mycobacterial hsp65 but not human hsp60, induces a significant retardation of the onset of adjuvant arthritis in Lewis rats. The data suggest that CH65 is a potential autoantigen involved in the pathogenesis of adjuvant arthritis in Lewis rats.

引用

页码：233 / 239

页数：7

共 30 条

[1]

van Eden W., Thole J.E.R., van der Zee R., Nordzij A., van Embden J.D.A., Hensen E.J., Cohen I.R., Cloning of the mycobacterial epitope recognized by T lymphocytes in adjuvant arthritis, Nature, 331, pp. 171-173, (1988)

[2]

Billingham M.E.J., Carney S., Butler R., Colston M.J., Mycobacterial A., 65 kD heat-shock protein induces antigen-specific suppression of adjuvant arthritis, but is not itself arthritoge-nic, J Exp Med, 171, pp. 339-344, (1990)

[3]

Yang X.D., Gasser J., Riniker B., Feige U., Treatment of adjuvant arthritis in rats: vaccination potential of a synthetic nonapeptide from the 65 kDa heat shock protein of mycobacteria, J Autoimmun, 3, pp. 11-23, (1990)

[4]

Yang X.D., Gasser J., Feige U., Prevention of adjuvant arthritis in rats by a nonapeptide from the 65 kDa mycobacterial heat-shock protein, Clin Exp Immunol, 81, pp. 189-194, (1990)

[5]

Yang X.D., Gasser J., Feige U., Prevention of adjuvant arthritis in rats by a nonapeptide from the 65 kDa mycobacterial heat-shock protein: specificity and mechanism, Clin Exp Immunol, 87, pp. 99-104, (1992)

[6]

Venner T.J., Gupta R.S., Nucleotide sequence of rat hsp60 (chaperonin, GroEL analog) cDNA, Nucleic Acids Res, 18, (1990)

[7]

Thole J.E., Keulen W.J., De-Bruyn J., Kolk A.H., Groothuis D.G., Berwald L.G., Tiesjema R.H., van Embden J.D., Characterization, sequence determination and immunogenicity of a 65 kDa protein of Mycobacterium bovis BCG expressed in E. coli K12, Infeclmmun, 55, pp. 1466-1475, (1987)

[8]

Feige U., Cohen I.R., The 65-kDa heat-shock protein in the pathogenesis, prevention and therapy of autoimmune arthritis and diabetes mellitus in rats and mice, Springer Semin Immunopathol, 13, pp. 99-113, (1991)

[9]

Van Eden W., Heat-shock proteins in autoimmune arthritis: a critical contribution based on the adjuvant arthritis model, APMIS, 98, pp. 383-394, (1990)

[10]

CoHen I.R., Autoimmunity to the chaperonins in the pathogenesis of arthritis and diabetes, Annu Rev Immunol, 9, pp. 567-589, (1991)

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