Successful cryopreservation of suspension cells by encapsulation-dehydration

被引：20

作者：

Bachiri, Y

Gazeau, C

Hansz, J

Morisset, C

Dereuddre, J

机构：

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1995年 / 43卷 / 03期

关键词：

alginate; dehydration; Catharanthus roseus; cell suspension; cryopreservation; encapsulation;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 0836 [生物工程]; 090102 [作物遗传育种]; 100705 [微生物与生化药学];

摘要：

Cryopreservation of a Catharanthus cell suspension was performed after encapsulation in alginate bends. Encapsulated cells were precultured in sucrose-enriched medium for several days, dried over silica gel, and directly cooled in liquid nitrogen. After rewarming in air at room temperature, alginate beads were placed on semi-solid culture medium. Following regrowth, beads transferred to liquid medium generated a new cell suspension. Cell survival and regrowth from cryopreserved encapsulated cells depended on preculture duration and residual water content after air-drying.

引用

页码：241 / 248

页数：8

共 38 条

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[2]

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[3]

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