POLYMERASE CHAIN-REACTION USING MIXED PRIMERS - CLONING OF HUMAN MONOCLONAL-ANTIBODY VARIABLE REGION GENES FROM SINGLE HYBRIDOMA CELLS

被引：117

作者：

LARRICK, JW ^{[1
]}

DANIELSSON, L ^{[1
]}

BRENNER, CA ^{[1
]}

WALLACE, EF ^{[1
]}

ABRAHAMSON, M ^{[1
]}

FRY, KE ^{[1
]}

BORREBAECK, CAK ^{[1
]}

机构：

[1] UNIV LUND,DEPT BIOTECHNOL,S-22100 LUND,SWEDEN

来源：

BIO-TECHNOLOGY | 1989年 / 7卷 / 09期

关键词：

D O I：

10.1038/nbt0989-934

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

引用

页码：934 / 938

页数：5

共 26 条

[1]

BANAPOUR B, 1987, J IMMUNOL, V139, P4027

[2] HUMAN MONOCLONAL-ANTIBODIES PRODUCED BY PRIMARY INVITRO IMMUNIZATION OF PERIPHERAL-BLOOD LYMPHOCYTES [J].

BORREBAECK, CAK ;

DANIELSSON, L ;

MOLLER, SA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (11) :3995-3999

[3] HUMAN MABS PRODUCED BY PRIMARY INVITRO IMMUNIZATION [J].

BORREBAECK, CAK .

IMMUNOLOGY TODAY, 1988, 9 (11) :355-359

[4]

BROWN BA, 1987, CANCER RES, V47, P3577

[5] CLUSTERING OF EXTENSIVE SOMATIC MUTATIONS IN THE VARIABLE REGION OF AN IMMUNOGLOBULIN HEAVY-CHAIN GENE FROM A HUMAN B-CELL LYMPHOMA [J].

CLEARY, ML ;

MEEKER, TC ;

LEVY, S ;

LEE, E ;

TRELA, M ;

SKLAR, J ;

LEVY, R .

CELL, 1986, 44 (01) :97-106

[6] ERRORS IN THE POLYMERASE CHAIN-REACTION [J].

DUNNING, AM ;

TALMUD, P ;

HUMPHRIES, SE .

NUCLEIC ACIDS RESEARCH, 1988, 16 (21) :10393-10393

[7] REPLACING THE COMPLEMENTARITY-DETERMINING REGIONS IN A HUMAN-ANTIBODY WITH THOSE FROM A MOUSE [J].

JONES, PT ;

DEAR, PH ;

FOOTE, J ;

NEUBERGER, MS ;

WINTER, G .

NATURE, 1986, 321 (6069) :522-525

[8]

Kabat E. A., 1987, SEQUENCES PROTEINS I, DOI 10.1016/0003-2697(84)90805-4

[9]

LARRICK JW, 1986, J BIOL RESP MODIF, V5, P379

[10] RAPID CLONING OF REARRANGED IMMUNOGLOBULIN GENES FROM HUMAN HYBRIDOMA CELLS USING MIXED PRIMERS AND THE POLYMERASE CHAIN-REACTION [J].

LARRICK, JW ;

DANIELSSON, L ;

BRENNER, CA ;

ABRAHAMSON, M ;

FRY, KE ;

BORREBAECK, CAK .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 160 (03) :1250-1256

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