EXPRESSION AND SECRETION OF THAUMATIN FROM ASPERGILLUS-ORYZAE

被引：28

作者：

HAHM, YT ^{[1
]}

BATT, CA ^{[1
]}

机构：

[1] CORNELL UNIV, DEPT FOOD SCI, ITHACA, NY 14853 USA

来源：

AGRICULTURAL AND BIOLOGICAL CHEMISTRY | 1990年 / 54卷 / 10期

关键词：

D O I：

10.1080/00021369.1990.10870381

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The plant protein thaumatin has been expressed and secreted in Aspergillus oryzae. The thaumatin II cDNA was expresed via a Saccharomyces cerevisiae glyceraldehyde 3-phosphate dehydrogenase (GPD) promoter, and its secretion is apparently directed into the medium by the native plant signal sequence. The S. cerevisiae GPD promoter is properly regulated in A. oryzae; the addition of glucose stimulates the production of thaumatin. A 22-kDa immunoreactive protein indistinguishable from mature thaumatin is present in the culture medium. A slightly larger, 25-kDa protein is observed in cell extracts and may represent the unprocessed form of the protein. Maximum accumulation of the extracellular immunoreactive protein is observed after two days and no further production is observed. © 1990 by the Japan Society for Bioscience, Biotechnology, and Agrochemistry.

引用

页码：2513 / 2520

页数：8

共 46 条

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