A COMPETITIVE ELISA FOR THE ANTI-INTERCELLULAR ADHESION MOLECULE-1 (ANTI-ICAM-1) BINDING-ACTIVITY OF MONOCLONAL-ANTIBODY R6.5 IN SERUM

被引：4

作者：

NORRIS, SH

JOHNSTONE, JN

DELEON, R

ROTHLEIN, R

机构：

[1] Boehringer Ingelheim Pharmaceuticals, Inc., Ridgefield, CT 06877

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1991年 / 9卷 / 03期

关键词：

COMPETITIVE ELISA; INTERCELLULAR ADHESION MOLECULE-1; R6.5 MOUSE MONOCLONAL ANTIBODY; SERUM EFFECTS; RABBIT PHARMACOKINETICS;

D O I：

10.1016/0731-7085(91)80149-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A competitive ELISA was developed to measure serum concentrations of immunoreactive R6.5, a mouse IgG2a monoclonal antibody which binds to and inhibits the interactions of human intercellular adhesion molecule-1 with glycoproteins of the CD18 complex and with rhinoviruses. The assay design permitted quantitative measurement of the functional activity of the antibody in serum. The working range of the assay was from 2.15 to 215 ng ml-1, and intra-assay and inter-assay relative standard deviations averaged 13 and 20%, respectively. Serum caused interferences with the assay when spiked in vitro, but authentic serum samples obtained from intravenously dosed animals did not exhibit these interferences. Characteristics of the assay, as well as results of a pharmacokinetic study in rabbits, are presented.

引用

页码：211 / 217

页数：7

共 14 条

[1]

BARTON RW, 1989, J IMMUNOL, V143, P1278

[2]

COSIMI AB, 1990, LEUKOCYTE ADHESION MOLECULES, P274

[3]

DITTMER DS, 1961, BLOOD OTHER BODY FLU, P5

[4] ANTIBODY-PRODUCTION BY HYBRIDOMAS [J].