MOLECULAR CHARACTERIZATION OF HELODERMIN-PREFERRING VIP RECEPTORS IN SUP-T1 LYMPHOMA-CELLS - EVIDENCE FOR RECEPTOR GLYCOSYLATION

被引：8

作者：

GOURLET, P ^{[1
]}

ROBBERECHT, P ^{[1
]}

CHRISTOPHE, J ^{[1
]}

机构：

[1] UNIV LIBRE BRUXELLES,SCH MED,DEPT BIOCHEM & NUTR,BLD WATERLOO 115,B-1000 BRUSSELS,BELGIUM

来源：

JOURNAL OF RECEPTOR RESEARCH | 1991年 / 11卷 / 05期

关键词：

D O I：

10.3109/10799899109064682

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Cross-linking of [I-125]helodermin to human SUP-T1 lymphoblasts with bis[2-(succinimidooxycarbonyloxy)ethyl]sulfone (BSOCOES) revealed a 63 K binding protein. This cross-linking was inhibited by helodermin and VIP. In cells submitted for 3-4 days to 0.2-mu-g/ml tunicamycin, the Mr of an increasing proportion of helodermin-preferring receptors was reduced to 50 K and the total number of receptors was decreased by about 50 %, without alteration in binding affinity and specificity. In parallel, the VIP-mediated adenylate cyclase stimulation was reduced by 30 % with no change in NaF-, Gpp[NH]p-, and PGE1 -stimulations. We conclude that a proper N-glycosylation of helodermin-preferring VIP receptors is required for normal receptor targeting and turnover but not for ligand binding and adenylate cyclase coupling.

引用

页码：831 / 848

页数：18

共 29 条

[1] HELODERMIN-VIP PREFERRING I-125 VIP BINDING-SITES DURING THE POSTNATAL-DEVELOPMENT OF THE PANCREAS [J].

BAWAB, W ;

FARJAUDON, N ;

LEMEUTH, V ;

CHASTRE, E ;

ROSSELIN, G ;

GUILLOTEAU, P ;

GESPACH, C .

REGULATORY PEPTIDES, 1989, 26 (02) :141-141

[2] VASOACTIVE INTESTINAL PEPTIDE AND ITS RECEPTORS IN FETUSES WITH CYSTIC-FIBROSIS [J].

CHASTRE, E ;

BAWAB, W ;

FAURE, C ;

EMAMI, S ;

MULLER, F ;

BOUE, A ;

GESPACH, C .

AMERICAN JOURNAL OF PHYSIOLOGY, 1989, 257 (04) :G561-G569

[3]

CHRISTOPHE JP, 1976, J BIOL CHEM, V251, P4629

[4] THE RAT-LIVER VASOACTIVE INTESTINAL PEPTIDE BINDING-SITE - MOLECULAR CHARACTERIZATION BY COVALENT CROSS-LINKING AND EVIDENCE FOR DIFFERENCES FROM THE INTESTINAL RECEPTOR [J].

COUVINEAU, A ;

LABURTHE, M .

BIOCHEMICAL JOURNAL, 1985, 225 (02) :473-479

[5] THE HUMAN VASOACTIVE INTESTINAL PEPTIDE RECEPTOR - MOLECULAR-IDENTIFICATION BY COVALENT CROSS-LINKING IN COLONIC EPITHELIUM [J].

COUVINEAU, A ;

LABURTHE, M .

JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1985, 61 (01) :50-55

[6] MOLECULAR-IDENTIFICATION AND STRUCTURAL REQUIREMENT OF VASOACTIVE INTESTINAL PEPTIDE (VIP) RECEPTORS IN THE HUMAN-COLON ADENOCARCINOMA CELL-LINE, HT-29 [J].

COUVINEAU, A ;

ROUSSET, M ;

LABURTHE, M .

BIOCHEMICAL JOURNAL, 1985, 231 (01) :139-143

[7] CHARACTERIZATION OF VASOACTIVE-INTESTINAL-PEPTIDE (VIP) RECEPTORS IN MAMMALIAN LUNG [J].

DICKINSON, KEJ ;

SCHACHTER, M ;

MILES, CMM ;

COY, DH ;

SEVER, PS .

PEPTIDES, 1986, 7 (05) :791-800

[8]

ELBATTARI A, 1988, ANN NY ACAD SCI, V527, P667

[9]

GEORGE ST, 1986, J BIOL CHEM, V261, P6559

[10] ADENYLATE-CYCLASE ACTIVATION BY VIP-HELODERMIN AND HISTAMINE H-2 RECEPTORS IN HUMAN THP-1 MONOCYTES MACROPHAGES - A POSSIBLE ROLE IN THE REGULATION OF SUPEROXIDE ANIONS PRODUCTION [J].

GESPACH, C ;

CHEDEVILLE, A ;

HURBAINKOSMATH, I ;

HOUSSET, B ;

DERENNE, JP ;

ABITA, JP .

REGULATORY PEPTIDES, 1989, 26 (02) :158-158

← 1 2 3 →