MEASUREMENT OF AFFINITY OF VIRAL MONOCLONAL-ANTIBODIES USING FAB'PEROXIDASE CONJUGATE - INFLUENCE OF ANTIBODY CONCENTRATION ON APPARENT AFFINITY

被引：10

作者：

AZIMZADEH, A

WEISS, E

VANREGENMORTEL, MHV

机构：

[1] CNRS,CNRS,INST BIOL MOLEC & CELLULAIRE,15 RUE DESCARTES,F-67084 STRASBOURG,FRANCE

[2] ECOLE SUPER BIOTECHNOL,STRASBOURG,FRANCE

来源：

MOLECULAR IMMUNOLOGY | 1992年 / 29卷 / 05期

关键词：

D O I：

10.1016/0161-5890(92)90196-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The binding affinity of a monoclonal antibody to tobacco mosaic virus (TMV) was studied using a Fab'-peroxidase conjugate. Measurement of the enzymatic activity allowed the determination of the amount of free antibody present after ultracentrifugation of virus-antibody complexes at equilibrium. The method was very sensitive and allowed measurements over a 1000-fold range of antibody concns. The calculated affinity constant decreased about 25 fold when the antibody concn used in the binding assay was increased from 30 ng/ml to 35-mu-g/ml.

引用

页码：601 / 608

页数：8

共 23 条

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