BACILLUS-THURINGIENSIS GROWTH, SPORULATION AND DELTA-ENDOTOXIN PRODUCTION IN OXYGEN LIMITED AND NONLIMITED CULTURES

被引:50
作者
AVIGNONEROSSA, C [1 ]
ARCAS, J [1 ]
MIGNONE, C [1 ]
机构
[1] NATL UNIV LA PLATA,FAC CIENCIAS EXACTAS,CTR INVEST & DESARROLLO FERMENTAC IND,CALLE 47 & 115,RA-1900 LA PLATA,ARGENTINA
关键词
BACILLUS-THURINGIENSIS; CELL YIELD; DELTA-ENDOTOXIN; OXYGEN; OXYGEN-LIMITED GROWTH;
D O I
10.1007/BF01201884
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The production of crystals and spores of Bacillus thuringiensis var. israelensis was studied under different aeration conditions. The results with 4 l batch cultures showed that for O2 non-limited cultures cell yield, toxin production and spore count were constant for all oxygen transfer rates (OTR). Under O2 limitation, delta-endotoxin concentrations and spore counts were lower than those obtained in non-limited cultures. In addition, delta-endotoxin yields diminished under O2 limitation, suggesting that the toxin synthesis mechanism could have been affected.
引用
收藏
页码:301 / 304
页数:4
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