ENHANCEMENT OF GROWTH OF RABBIT CORNEAL ENDOTHELIAL-CELLS BY PDGF

被引：3

作者：

KAMIYAMA, K ^{[1
]}

IGUCHI, I ^{[1
]}

WANG, XG ^{[1
]}

KITA, M ^{[1
]}

IMANISHI, J ^{[1
]}

YAMAGUCHI, N ^{[1
]}

HONGO, M ^{[1
]}

SOTOZONO, C ^{[1
]}

KINOSHITA, S ^{[1
]}

机构：

[1] KYOTO PREFECTURAL UNIV MED,DEPT MICROBIOL,KAMIKYO KU,KYOTO 602,JAPAN

来源：

CORNEA | 1995年 / 14卷 / 02期

关键词：

CORNEA; ENDOTHELIAL CELLS; PLATELET-DERIVED GROWTH FACTOR (PDGF); CELL GROWTH; PDGF RECEPTOR;

D O I：

暂无

中图分类号：

R77 [眼科学];

学科分类号：

100212 [眼科学];

摘要：

The effect of platelet-derived growth factor (PDGF) on the growth of rabbit corneal endothelial cells and the expression of PDGF receptor mRNA in these cells were examined in the present study. When rabbit corneal endothelial cells were cultured in the presence of natural PDGF, recombinant PDGF-BB, and PDGF-AA for 1, 3, and 5 days, natural PDGF and recombinant PDGF-BB enhanced the growth of the endothelial cells, but PDGF-AA did not. This growth promotion was suppressed by anti-PDGF antibody. PDGF enhanced also the incorporation of SH-thymidine into the cells. When PDGF was combined with epidermal growth factor (EGF), the additive effect was recognized. The expression of the PDGF receptor mRNA was found in the endothelial cells by the polymerase chain reaction (PCR) method. It was concluded from these results that PDGF enhanced the growth of the corneal endothelial cells and that the PDGF receptor mRNA was expressed in these cells. These results suggest that PDGF may regulate corneal endothelial cell growth.

引用

页码：187 / 195

页数：9