AN INHIBITORY ROLE FOR PHOSPHATIDYLINOSITOL 3-KINASE IN INSULIN-SECRETION FROM PANCREATIC B-CELL LINE MIN6

被引：42

作者：

HAGIWARA, S

SAKURAI, T

TASHIRO, F

HASHIMOTO, Y

MATSUDA, Y

NONOMURA, Y

MIYAZAKI, J

机构：

[1] UNIV TOKYO,FAC MED,DEPT DIS RELATED GENE REGULAT RES SANDOZ,TOKYO 113,JAPAN

[2] UNIV TOKYO,FAC MED,DEPT PHARMACOL 1,TOKYO 113,JAPAN

[3] UNIV TOKYO,FAC MED,DEPT INTERNAL MED 1,TOKYO 113,JAPAN

[4] KYOWA HAKKO KOGYO CO LTD,TOKYO RES LABS,TOKYO 194,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 214卷 / 01期

关键词：

D O I：

10.1006/bbrc.1995.2255

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phosphatidylinositol 3-kinase (PI3-kinase) has been implicated in the regulation of vesicular transport. We examined the roles of PI3-kinase in the glucose-induced insulin secretion from the pancreatic beta cell line MIN6 by using wortmannin, a potent inhibitor of PI3-kinase. Low concentrations of wortmannin markedly potentiated the glucose-induced insulin secretion. This effect was probably mediated by PI3-kinase inhibition. Furthermore, wortmannin completely canceled the suppressive effect of insulin-like growth factor-I on insulin secretion from MIN6 cells. On the basis of these results, we discuss a possible role of PI3-kinase in the negative feed-back regulation of insulin secretion. (C) 1995 Academic Press, Inc.

引用

页码：51 / 59

页数：9

共 19 条

[1] WORTMANNIN IS A POTENT PHOSPHATIDYLINOSITOL 3-KINASE INHIBITOR - THE ROLE OF PHOSPHATIDYLINOSITOL 3,4,5-TRISPHOSPHATE IN NEUTROPHIL RESPONSES [J].