STUDIES ON PROTEINASES FROM CALOTROPIS-GIGANTEA LATEX .1. PURIFICATION AND SOME PROPERTIES OF 2 PROTEINASES CONTAINING CARBOHYDRATE

被引：44

作者：

ABRAHAM, KI

JOSHI, PN

机构：

[1] Biochemistry Division, Department of Chemistry, University of Poona, Poona

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 568卷 / 01期

关键词：

(Calotropis gigantea); Calotropain; Glycoproteinase; Proteinase;

D O I：

10.1016/0005-2744(79)90278-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two proteinase containing carbohydrate, called calotropain-F1 and calotropain-FII, were purified from Calotropis gigantea latex by CM-Sephadex C-50 chromatography. Both calotropain-FI and FII were found to be homogeneous by rechromatography on CM-Sephadex C-50, gel filtration on Sephadex G-100, electrophoresis on polyacrylamide gel and by N-terminal amino acid analysis. Some properties of these enzymes are reported. © 1979.

引用

页码：111 / 119

页数：9

共 37 条

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[2]

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[3]

BOSE S. M., 1958, ENZYMOLOGIA, V19, P186

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