FORMATION OF 7-CIS-RETINAL AND 13-CIS-RETINAL PIGMENTS BY IRRADIATING SQUID RHODOPSIN

被引:31
作者
MAEDA, A
SHICHIDA, Y
YOSHIZAWA, T
机构
[1] Department of Biophysics, Faculty of Science, Kyoto University
关键词
D O I
10.1021/bi00575a010
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Squid rhodopsin was irradiated with orange light (>530 nm) at various temperatures from -190 to 10 °C until a photo-steady-state mixture was formed. Then the chromophoric retinals were extracted from the photo-steady-state mixtures and their isomer composition was analyzed by high-performance liquid chromatography. In the case of a photo-steady-state mixture formed at -85 °C, large peaks in the chromatogram were found at the positions of both 1-cis-and 13-cw-retinals. Each peak was further identified by synthesizing the pigments from these retinals with cattle opsin or apobacteriorhodopsin. Both 7-cis- and 13-m-retinals were also extracted from a photo-steady-state mixture formed by irradiation at -40, at 0, or at 10 °C. These isomers were scarcely detected in a photo-steady-state mixture formed by irradiation at -190 °C, though 9-cis-retinal was found as a major constituent in this mixture. Irradiation of lumirhodopsin at -190 °C, however, produced 7-cis-retinal pigment. These findings suggest that bathorhodopsin may have a conformation to prevent the formation of 7-cis-retinal from the all-trans form and that this particular conformation may be relaxed by the conversion of bathorhodopsin to lumirhodopsin. © 1979, American Chemical Society. All rights reserved.
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页码:1449 / 1453
页数:5
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