SELECTIVE FLOCCULATION OF NUCLEIC-ACIDS, LIPIDS, AND COLLOIDAL PARTICLES FROM A YEAST-CELL HOMOGENATE BY POLYETHYLENEIMINE, AND ITS SCALE-UP

被引：46

作者：

MILBURN, P ^{[1
]}

BONNERJEA, J ^{[1
]}

HOARE, M ^{[1
]}

DUNNILL, P ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL,DEPT CHEM & BIOCHEM ENGN,SERC,CTR BIOCHEM ENGN,TORRINGTON PL,LONDON WC1E 7JE,ENGLAND

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 1990年 / 12卷 / 07期

关键词：

borax; cell debris; lipid; nucleic acid; Polyethyleneimine; protein;

D O I：

10.1016/0141-0229(90)90070-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Nucleic acids, lipid, and colloidal particulate material can be selectively flocculated from a yeast cell homogenate by the cationic polymer polyethyleneimine (PEI). Flocculation can occur from a crude homogenate, a homogenate clarified centrifugally, or by the prior use of sodium tetraborate (borax). Flocculation from a homogenate previously clarified by the use of borax is best suited for large-scale operation. The supernatant obtained following centrifugation is effectively free of nucleic acid, lipid, and particulate material with essentially 100% soluble enzyme recovery. Enzyme specific activity increases by approximately 45% compared to a zero PEI control. © 1990.

引用

页码：527 / 532

页数：6

共 19 条

[1] PRECIPITATION OF NUCLEIC-ACIDS WITH POLYETHYLENEIMINE AND CHROMATOGRAPHY OF NUCLEIC-ACIDS AND PROTEINS ON IMMOBILIZED POLYETHYLENEIMINE [J].