A NEW, HIGHLY SENSITIVE METHOD FOR THE DETECTION AND QUANTIFICATION OF PENICILLIN-BINDING PROTEINS

被引：28

作者：

GALLENI, M

LAKAYE, B

LEPAGE, S

JAMIN, M

THAMM, I

JORIS, B

FRERE, JM

机构：

[1] UNIV LIEGE,INST CHIM,ENZYMOL LAB,B6,B-4000 SART,BELGIUM

[2] UNIV LIEGE,INST CHIM,CTR INGN PROT,B-4000 SART,BELGIUM

来源：

BIOCHEMICAL JOURNAL | 1993年 / 291卷

关键词：

D O I：

10.1042/bj2910019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for the identification and quantification of penicillin-binding proteins is described which uses fluorescein-coupled penicillins. It allows the rapid detection of 0.2 pmol with the naked eye and 2 fmol with the help of an A.L.F. automatic DNA sequencer. Direct labelling can also be performed on whole bacterial cells.

引用

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页码：19 / 21

页数：3

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