QUANTITATIVE MEASUREMENT OF CYCLOSPORINE-A IN BLOOD BY TIME-OF-FLIGHT MASS-SPECTROMETRY

被引:97
作者
MUDDIMAN, DC
GUSEV, AI
PROCTOR, A
HERCULES, DM
VENKATARAMANAN, R
DIVEN, W
机构
[1] UNIV PITTSBURGH, DEPT CHEM, PITTSBURGH, PA 15260 USA
[2] UNIV PITTSBURGH, DEPT PHARMACEUT SCI, PITTSBURGH, PA 15213 USA
[3] UNIV PITTSBURGH, DEPT PATHOL, PITTSBURGH, PA 15213 USA
关键词
D O I
10.1021/ac00086a023
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Analytical methods have been developed for the detection and quantification of cyclosporin A (CsA) in blood using time-of-flight secondary-ion mass spectrometry (TOF-SIMS) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS). Linear calibration curves were obtained for both methods in the range 25-2000 ng/mL in whole blood. The limit of detection (LOD) and limit of quantification (LOQ) were determined to be 7 and 23 ng/mL, respectively, for the TOF-SIMS method; the LOD and LOQ were 10 and 33 ng/mL, respectively, for the MALDI/TOF-MS method. Coefficients of variation ranged from 3 to 5% for the TOF-SIMS method and from 4 to 8% for the MALDI/TOF-MS method. Blood samples were also analyzed by a nonspecific method, fluorescence polarization immunoassay (FIPA), and by a specific method, high-performance liquid chromatography (HPLC). The TOF-MS results are in good agreement with the HPLC results, but not with the FPIA results. The TOF-MS methods can also provide information about CsA metabolites.
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页码:2362 / 2368
页数:7
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