POLYPEPTIDES OF INFLUENZA VIRUS .I. CYTOPLASMIC SYNTHESIS AND NUCLEAR ACCUMULATION

Three major structural peptides were found in radioactive influenza virus type A Bel that had been grown in calf kidney cells, purified, and analyzed by acrylamide gel electrophoresis. The same three peptides were found in infected HeLa cells. All proved to be stable; none was a precursor of another. The site of synthesis and subsequent migration of these peptides was investigated by cell fractionation. Evidence is presented which suggests that viral peptides were synthesized in the cytoplasm, and that two of them subsequently underwent significant accumulation into the nucleus. © 1969.