AUTOMATED-DETERMINATION OF TOTAL PLASMA-CHOLESTEROL - SERUM CALIBRATION TECHNIQUE

被引：34

作者：

BACHORIK, PS

WOOD, PDS

WILLIAMS, J

KUCHMAK, M

AHMED, S

LIPPEL, K

ALBERS, J

机构：

[1] STANFORD UNIV,SCH MED,DEPT MED,STANFORD,CA 94305

[2] CTR DIS CONTROL,LIPIDS SECT,ATLANTA,GA 30333

[3] UNIV WASHINGTON,SCH MED,DEPT BIOCHEM,SEATTLE,WA 98195

[4] GEORGETOWN UNIV,SCH MED,WASHINGTON,DC 20007

[5] NHLBI,BETHESDA,MD 20014

[6] UNIV WASHINGTON,SCH MED,DEPT MED,SEATTLE,WA 98195

来源：

CLINICA CHIMICA ACTA | 1979年 / 96卷 / 1-2期

关键词：

D O I：

10.1016/0009-8981(79)90064-0

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The automated (AutoAnalyzer II) determination of cholesterol in nine serum pools in the concentration range 3.465-8.871 mmol/1, gave results that were approximately 10% higher than reference values when the analyses were based on unesterified cholesterol standards containing the same amount of water as the sample extracts (1963 analyses in 12 laboratories during a 12 month period; automated value = 0.032 + 1.10 × (Reference value)). A serum calibration procedure was successful in correcting the observed serum pool values to within +0.2 to -2.1% of reference values, and was equally effective in correcting the values observed for aliquots of 368 fresh-frozen plasma samples analyzed in each of the 12 laboratories during a 38 month period. © 1979.

引用

页码：145 / 153

页数：9

共 13 条

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[2] Bachorik P. S., 1978, Protides of the biological fluids, P307
[3] BACHORIK PS, 1977, HYPERLIPIDEMIA DIAGN, P41
[4] FREDRICKSON DS, 1972, METABOLIC BASIS INHE, P545
[5] GJONE E, 1978, METABOLIC BASIS INHE, P589
[6] KUCHMAK M, Patent No. 3764556
[7] LAROSA JC, 1977, HYPERLIPIDEMIA DIAGN, P205
[8] Tonks D., 1967, CLIN BIOCHEM, V1, P12, DOI [10.1016/S0009-9120(67)80002-X, DOI 10.1016/S0009-9120(67)80002-X]
[9] DIRECT ESTIMATION OF ESTERIFIED CHOLESTEROL WITH A NUMBER OF COMMONLY USED CHOLESTEROL COLOUUR REAGENTS
WEBSTER, D
[J]. CLINICA CHIMICA ACTA, 1963, 8 (05) : 731 - &
[10] WILLIAMS J, 1973, CLIN CHEM, V19, P650

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