TRANSLATIONAL MODIFICATION OF AN 18 KILODALTON POLYPEPTIDE BY SPERMIDINE IN RICE CELL-SUSPENSION CULTURES

被引：24

作者：

MEHTA, AM ^{[1
]}

SAFTNER, RA ^{[1
]}

SCHAEFFER, GW ^{[1
]}

MATTOO, AK ^{[1
]}

机构：

[1] USDA ARS, BELTSVILLE AGR RES CTR, PLANT HORMONE LAB, BELTSVILLE, MD 20705 USA

来源：

PLANT PHYSIOLOGY | 1991年 / 95卷 / 04期

关键词：

D O I：

10.1104/pp.95.4.1294

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

When rice (Oryza sativa) cell suspension cultures are grown in the presence of [terminal methylenes-H-3]spermidine, label is incorporated in a single polypeptide with a molecular mass of 18 kilodaltons on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Preincubation of cell cultures with polyamine biosynthesis inhibitors difluoromethylarginine and difluoromethylornithine, resulted in increased incorporation of the label into the 18 kilodalton polypeptide. In cells in which protein synthesis was arrested by cycloheximide, no label was detected in the 18 kilodalton polypeptide, suggesting a requirement for de novo protein synthesis.

引用

页码：1294 / 1297

页数：4

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