RECONSTITUTION OF A PURIFIED MU-OPIOID BINDING-PROTEIN IN LIPOSOMES - SELECTIVE, HIGH-AFFINITY, GTP-GAMMA-S-SENSITIVE MU-OPIOID AGONIST BINDING IS RESTORED

被引:10
作者
GIOANNINI, TL
FAN, LQ
HYDE, L
OFRI, D
YAO, YH
HILLER, JM
SIMON, EJ
机构
[1] NYU MED CTR,DEPT PSYCHIAT,NEW YORK,NY 10016
[2] NYU MED CTR,DEPT PHARMACOL,NEW YORK,NY 10016
[3] CUNY BERNARD M BARUCH COLL,DEPT NAT SCI,NEW YORK,NY 10010
关键词
D O I
10.1006/bbrc.1993.1906
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An opioid binding protein (GBP) purified to homogeneity from bovine striatal membranes has been reconstituted in liposomes. The liposomes were produced by PEG-precipitation of OBP in the presence of a CHAPS extract of bovine striatum, devoid of opioid binding. High affinity μ-agonist binding was restored. The binding was selective for μ-agonists, stereospecific and inhibited by GTPγS. These results demonstrate that there is recoupling of OBP with G-protein and confirm our earlier evidence that the purified OBP is a μ-opioid binding site. © 1993 Academic Press, Inc.
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收藏
页码:901 / 908
页数:8
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