FLOW CYTOMETRIC INDIRECT IMMUNOFLUORESCENCE ASSAY WITH HIGH-SENSITIVITY AND SPECIFICITY FOR THE DETECTION OF ANTIBODIES TO HSV-1 AND HSV-2

被引：2

作者：

CARUSO, A

TINTI, M

PERONI, L

CABIBBO, E

DERANGO, C

MANCA, N

TURANO, A

机构：

[1] Institute of Microbiology, University of Brescia, Brescia, 1 - 25123, zzale Ospedali Civili

来源：

EUROPEAN JOURNAL OF EPIDEMIOLOGY | 1993年 / 9卷 / 05期

关键词：

FLOW CYTOMETRY; IMMUNOFLUORESCENCE; ANTIBODIES; HSV-1; HSV-2;

D O I：

10.1007/BF00209534

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Cells infected with HSV-1 or HSV-2 develop viral antigens which can be detected by immunofluorescence. We developed a flow cytometric indirect immunofluorescence assay to detect and quantitate antibodies to HSV-1 and HSV-2 in human sera. Results obtained by flow cytometry for detecting antibodies against HSV-1, when compared with results obtained by ELISA, showed an index of overall agreement of 100%. The correlation between the antibody titers obtained with each method was found to be highly significant. An index of overall agreement equal to 94.1% was observed between results obtained by flow cytometry and by immunofluorescence as concerns the discrimination of HSV-2 positive from negative samples. However, the correlation between antibody titers was found to be not statistically significant. The flow cytometric assay proved to be type-specific.

引用

页码：547 / 552

页数：6

共 28 条

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