A HIGHLY SENSITIVE CHROMOGENIC MICROPLATE ASSAY FOR QUANTIFICATION OF RAT AND HUMAN PLASMINOGEN

被引：7

作者：

KULSETH, MA ^{[1
]}

HELGELAND, L ^{[1
]}

机构：

[1] UNIV OSLO,DEPT BIOCHEM,POB 1041,N-0316 OSLO,NORWAY

来源：

ANALYTICAL BIOCHEMISTRY | 1993年 / 210卷 / 02期

关键词：

D O I：

10.1006/abio.1993.1201

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and highly sensitive chromogenic microplate assay for quantification of rat and human plasminogen in plasma samples and subcellular fractions has been developed. The assay is based on a conversion of plasminogen to plasmin, using urokinase as an activator, and a subsequent cleavage of achromogenic plasmin substrate D-alanyl-L-cyclohexylalanyl-L-lysine-p-nitroanilide-dihydroacetate. p-Nitroaniline being released by the cleavage is then measured at 410 nm with a microplate reader. The assay includes an acidification step to make plasminogen more readily activated to plasmin. The method is suitable for analyses of a large number of samples, measuring plasminogen in the nanogram range (0.5-50 ng/50 μl of sample). © 1994 Academic Press, Inc. All rights reserved.

引用

页码：314 / 317

页数：4

共 13 条

[1] PLASMINOGEN IS SYNTHESIZED BY PRIMARY CULTURES OF RAT HEPATOCYTES [J].