MICROENCAPSULATION OF YEAST-CELLS IN THE CALCIUM ALGINATE MEMBRANE

被引：52

作者：

CHEONG, SH

PARK, JK

KIM, BS

CHANG, HN

机构：

[1] KOREA ADV INST SCI & TECHNOL,DEPT CHEM ENGN,TAEJON 305701,SOUTH KOREA

[2] KYUNGPOOK NATL UNIV,DEPT CHEM ENGN,TAEGU 702701,SOUTH KOREA

[3] KOREA ADV INST SCI & TECHNOL,BIOPROC ENGN RES CTR,TAEJON 305701,SOUTH KOREA

来源：

BIOTECHNOLOGY TECHNIQUES | 1993年 / 7卷 / 12期

关键词：

D O I：

10.1007/BF00156366

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cells of Saccharomyces cerevisiae (ATCC 24858) were encapsulated in the calcium alginate membrane and cultured. Swelling of the capsule was prevented by adding 0.2 g CaCl2 to 1 L growth medium. The dry cell concentration based on the inner volume of the capsule reached 309 g/L, which was much higher than could be obtained by cell entrapment. Ah the cells remained inside the capsule during the cultivation. The flux of CO2 through the capsule membrane increased approximately twice by adding a nonionic surfactant to the CaCl2 solution during the step of capsule formation.

引用

页码：879 / 884

页数：6

共 12 条

[1]

Chang T. M. S., 1972, ARTIFICIAL CELLS

[2] PHYSICAL STUDIES ON CELL IMMOBILIZATION USING CALCIUM ALGINATE GELS [J].