TRACE ANALYSIS OF PROTEIN AMINO ACIDS AS N(O,S)-ISOBUTYLOXYCARBONYL TERT.-BUTYLDIMETHYLSILYL DERIVATIVES IN AQUEOUS SAMPLES.

被引：6

作者：

Kim, Kyoung-Rae ^{[1
]}

Kim, Jung-Han ^{[2
]}

Oh, Chang-Hwan ^{[2
]}

机构：

[1] Sungkyunkwan Univ, Coll Pharm, Suwon 440746, South Korea

[2] Yonsei Univ, Dept Food Engn, Seoul 120749, South Korea

来源：

ANALYTICAL SCIENCES | 1991年 / 7卷

关键词：

amino acids; derivatizaiton; solid-phase extraction; GC analysis;

D O I：

10.2116/analsci.7.Supple_231

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

N(O,S)-isoButyloxycarbonyl(-isoBOC) derivatization with subsequent solid -phase extraction and silylation was investigated for the trace analysis of protein amino acids in complex aqueous samples. The amino acids in basic aqueous medium were allowed to react with isobutyl chloroformate. After acidification the resulting N(O,S)-isoBOC amino acids were solid-phase extracted using Chromosorb P as solid sorbent and then converted to tert.-butyldimethylsily1 derivatives, followed by direct gas chromatographic analysis. The application of the method to the amino acid profiling of various complex samples is demonstrated.

引用

页码：231 / 234

页数：4

共 7 条

[1]

DASNEVES HJC, 1987, J CHROMATOGR, V392, P249, DOI 10.1016/S0021-9673(01)94270-0

[2] CAPILLARY GAS-CHROMATOGRAPHIC ANALYSIS OF AMINO-ACIDS IN BLOOD AND PROTEIN HYDROLYSATES AS TERT-BUTYLDIMETHYLSILYL DERIVATIVES [J].

EARLY, RJ ;

THOMPSON, JR ;

SEDGWICK, GW ;

KELLY, JM ;

CHRISTOPHERSON, RJ .

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1987, 416 (01) :15-23

[3]

Kim K. R., 1989, P 3 KOR JAP JOINT S, P119

[4]

Kim K. R., 1991, J CHROMATOGR UNPUB

[5] SIMULTANEOUS GAS-CHROMATOGRAPHY OF VOLATILE AND NONVOLATILE CARBOXYLIC-ACIDS AS TERT-BUTYLDIMETHYLSILYL DERIVATIVES [J].

KIM, KR ;

HAHN, MK ;

ZLATKIS, A ;

HORNING, EC ;

MIDDLEDITCH, BS .

JOURNAL OF CHROMATOGRAPHY, 1989, 468 :289-301

[6] ANALYSIS OF AMINO-ACIDS BY GAS-LIQUID-CHROMATOGRAPHY AS TERT-BUTYLDIMETHYLSILYL DERIVATIVES - PREPARATION OF DERIVATIVES IN A SINGLE REACTION [J].

MACKENZIE, SL ;

TENASCHUK, D ;

FORTIER, G .

JOURNAL OF CHROMATOGRAPHY, 1987, 387 :241-253

[7] GAS-LIQUID-CHROMATOGRAPHIC ANALYSIS OF PROTEIN AMINO-ACIDS AS N-ISOBUTYLOXYCARBONYLAMINO ACID METHYL-ESTERS [J].

MAKITA, M ;

YAMAMOTO, S ;

KONO, M .

JOURNAL OF CHROMATOGRAPHY, 1976, 120 (01) :129-140

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