ANALYSIS OF ACTIVE ANTIBODY CONCENTRATION - SEPARATION OF AFFINITY AND CONCENTRATION PARAMETERS

被引：114

作者：

KARLSSON, R

FAGERSTAM, L

NILSHANS, H

PERSSON, B

机构：

[1] Pharmacia Biosensor AB

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1993年 / 166卷 / 01期

关键词：

CONCENTRATION; AFFINITY; MASS TRANSFER; (ANTIBODY);

D O I：

10.1016/0022-1759(93)90330-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Antibody binding to surfaces with differing amounts of immobilised antigen was measured in a biosensor system using surface plasmon resonance detection. Binding rates obtained during the initial binding phase on high density antigen surfaces were proportional to antibody concentration and independent of antigen-antibody affinity. One antibody calibration curve covering the range from 0.5 to 160 nM (0.08-25 mu g/ml) antibody was valid for IgG antibodies with different antigen specificities. To illustrate the use of this methodology active antibody concentrations were analysed in culture media and in rabbit serum.

引用

页码：75 / 84

页数：10

共 17 条

[1]

Fagerstam L G, 1990, J Mol Recognit, V3, P208, DOI 10.1002/jmr.300030507

[2] BIOSPECIFIC INTERACTION ANALYSIS USING SURFACE-PLASMON RESONANCE DETECTION APPLIED TO KINETIC, BINDING-SITE AND CONCENTRATION ANALYSIS [J].