A FIBER-BED BIOREACTOR FOR ANCHORAGE-DEPENDENT ANIMAL-CELL CULTURES .2. SCALEUP POTENTIAL

被引：17

作者：

MURAKAMI, S ^{[1
]}

CHIOU, TW ^{[1
]}

WANG, DIC ^{[1
]}

机构：

[1] MIT,CTR BIOTECHNOL PROC ENGN,CAMBRIDGE,MA 02139

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1991年 / 37卷 / 08期

关键词：

ANIMAL CELL CULTURE; BIOREACTOR; AIRLIFT; FIBER-BED BIOREACTOR; OXYGEN TRANSFER;

D O I：

10.1002/bit.260370811

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A simple hydrodynamic model is introduced to describe the airlift fiber-bed bioreactor, which can enhance the volumetric productivity of anchorage-dependent animal cell cultures. By applying the model, liquid flow rates and volumetric mass transfer coefficients are predicted and are in agreement with experimental measurements. Consequently, the optimal reactor configuration giving the maximal oxygen supply is derived. Also, theoretical scaleup potential of this concentric internal loop reactor is considered for volumes ranging from 10 to 67,000 L with which cell densities of 5.1 X 10(7) and 1.2 X 10(7) cells/cm3, respectively, can be maintained.

引用

页码：762 / 769

页数：8

共 17 条

[1] GAS HOLDUP AND OVERALL VOLUMETRIC OXYGEN-TRANSFER COEFFICIENT IN AIRLIFT CONTACTORS [J].