BETA-AMYLOID PRECURSOR PROTEIN OF ALZHEIMERS-DISEASE IN CULTURED BOVINE OLIGODENDROCYTES

被引：13

作者：

MIZUGUCHI, M

IKEDA, K

KIM, SU

机构：

[1] UNIV BRITISH COLUMBIA, DEPT MED, DIV NEUROL, 2211 WESBROOK MALL, VANCOUVER V6T 1W5, BC, CANADA

[2] PSYCHIAT RES INST TOKYO, DIV ULTRASTRUCT & HISTOCHEM, TOKYO, JAPAN

来源：

JOURNAL OF NEUROSCIENCE RESEARCH | 1992年 / 32卷 / 01期

关键词：

AMYLOID; IMMUNOCYTOCHEMISTRY; IMMUNOCHEMISTRY; OLIGODENDROCYTE; TISSUE CULTURE;

D O I：

10.1002/jnr.490320105

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The production of beta-amyloid precursor protein (beta-APP) in cultured oligodendrocytes isolated from adult bovine brains was examined by immunohistochemistry and immunoblotting. Immunostaining of oligodendrocytes with antibodies specific for the carboxy terminus of beta-APP demonstrated positive immunoreactivity of oligodendroglial cytoplasm. Immunoblot analysis of cellular extracts detected two distinct bands with estimated molecular weight of 118 and 105 kDa. The amount of these beta-APP subspecies increased considerably in response to their attachment to the poly-L-lysine substratum.

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页码：34 / 42

页数：9

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