CHARACTERIZATION OF HUMAN DEOXYCYTIDINE KINASE - CORRELATION WITH CDNA SEQUENCES

被引：9

作者：

ERIKSSON, S ^{[1
]}

CEDERLUND, E ^{[1
]}

BERGMAN, T ^{[1
]}

JORNVALL, H ^{[1
]}

BOHMAN, C ^{[1
]}

机构：

[1] KAROLINSKA INST,DEPT CHEM 1,S-10401 STOCKHOLM 60,SWEDEN

来源：

FEBS LETTERS | 1991年 / 280卷 / 02期

关键词：

DEOXYCYTIDINE KINASE; PEPTIDE SEQUENCE; AMINO ACID COMPOSITION; CDNA SEQUENCE;

D O I：

10.1016/0014-5793(91)80332-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Existing data on the structure of human deoxycytidine kinase (dCK) diverge. A monomeric 60 kDa form has been isolated and the cloning of a cDNA coding for 626 amino acids corresponding to a 71 kDa protein has been reported. However, pure dCK isolated from leukemic spleen is a dimer of 30 kDa subunits. Amino acid sequences of peptides from digests of this protein are now presented. None of the peptide structures obtained correspond to the cDNA for the 71 kDa protein, but to a for a 30.5 kDa dCK recently cloned. Furthermore, homology of the peptide sequences of dCK to parts of thymidine kinases and protein-tyrosine kinases are detected.

引用

页码：363 / 366

页数：4

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