EXPRESSION OF THE FIBROBLAST GROWTH-FACTOR RECEPTOR FGFR-1/FLG DURING GASTRULATION AND SEGMENTATION IN THE MOUSE EMBRYO

被引:140
作者
YAMAGUCHI, TP [1 ]
CONLON, RA [1 ]
ROSSANT, J [1 ]
机构
[1] MT SINAI HOSP,SAMUEL LUNENFELD RES INST,TORONTO M5G 1X5,ONTARIO,CANADA
基金
英国医学研究理事会;
关键词
D O I
10.1016/0012-1606(92)90157-C
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Recent evidence from studies in both amphibians and mammals suggest that the fibroblast growth factor (FGF) family of signaling molecules and their receptors may play regulatory roles during early embryogenesis. We have used both standard and whole-mount in situ hybridization techniques to analyze the temporal and spatial expression patterns of the murine fibroblast growth factor receptor-1 (FGFR-1) in order to help define the role of FGFs in the processes of gastrulation and segmentation. FGFR-1 transcripts were detected in the primitive ectoderm of the egg cylinder embryo but not in the primitive endoderm or ectoplacental cone. During gastrulation, FGFR-1 mRNA were expressed at high levels in the migrating embryonic mesoderm of the mid-streak-stage embryo. Late-streak-stage embryos displayed strong expression in both the embryonic ectoderm and mesoderm. Within the ectodermal lineage, FGFR-1 mRNA later became localized to the neural ectoderm during its formation and continued to be expressed at high levels throughout neural development. In the mesodermal lineage, FGFR-1 transcripts became concentrated in the posterior medial mesoderm of the embryo as it condensed to form paraxial mesoderm. The most striking expression patterns were observed before and during segmentation when FGFR-1 was strongly expressed in the presomitic mesoderm and the rostral half of the newly formed somites. The patterns of expression are consistent with a role for FGFR-1 in posterior mesoderm formation. FGFR-1 may also play significant roles in the formation of neural ectoderm and the early events that establish compartments within the developing somites. © 1992.
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页码:75 / 88
页数:14
相关论文
共 71 条
  • [1] ALTABA AR, 1989, NATURE, V341, P33
  • [2] ALTABA ARI, 1991, TRENDS GENET, V7, P276
  • [3] EXPRESSION OF A DOMINANT NEGATIVE MUTANT OF THE FGF RECEPTOR DISRUPTS MESODERM FORMATION IN XENOPUS EMBRYOS
    AMAYA, E
    MUSCI, TJ
    KIRSCHNER, MW
    [J]. CELL, 1991, 66 (02) : 257 - 270
  • [4] A MAMMALIAN PROTEIN-KINASE WITH POTENTIAL FOR SERIN THREONINE AND TYROSINE PHOSPHORYLATION IS RELATED TO CELL-CYCLE REGULATORS
    BENDAVID, Y
    LETWIN, K
    TANNOCK, L
    BERNSTEIN, A
    PAWSON, T
    [J]. EMBO JOURNAL, 1991, 10 (02) : 317 - 325
  • [5] EXPRESSION OF 2 DIFFERENT FORMS OF FIBROBLAST GROWTH-FACTOR RECEPTOR-1 IN DIFFERENT MOUSE-TISSUES AND CELL-LINES
    BERNARD, O
    LI, M
    REID, HH
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (17) : 7625 - 7629
  • [6] BRUNETTI A, 1990, J BIOL CHEM, V265, P5960
  • [7] THE HEPARIN-BINDING (FIBROBLAST) GROWTH-FACTOR FAMILY OF PROTEINS
    BURGESS, WH
    MACIAG, T
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 : 575 - 606
  • [8] THE PROTO-ONCOGENE C-KIT ENCODING A TRANSMEMBRANE TYROSINE KINASE RECEPTOR MAPS TO THE MOUSE W-LOCUS
    CHABOT, B
    STEPHENSON, DA
    CHAPMAN, VM
    BESMER, P
    BERNSTEIN, A
    [J]. NATURE, 1988, 335 (6185) : 88 - 89
  • [9] DIFFERENTIAL ACTIVATION OF XENOPUS HOMEO BOX GENES BY MESODERM-INDUCING GROWTH-FACTORS AND RETINOIC ACID
    CHO, KWY
    DEROBERTIS, EM
    [J]. GENES & DEVELOPMENT, 1990, 4 (11) : 1910 - 1916
  • [10] CLONING AND EXPRESSION OF 2 DISTINCT HIGH-AFFINITY RECEPTORS CROSS-REACTING WITH ACIDIC AND BASIC FIBROBLAST GROWTH-FACTORS
    DIONNE, CA
    CRUMLEY, G
    BELLOT, F
    KAPLOW, JM
    SEARFOSS, G
    RUTA, M
    BURGESS, WH
    JAYE, M
    SCHLESSINGER, J
    [J]. EMBO JOURNAL, 1990, 9 (09) : 2685 - 2692