REPLACEMENT OF SULFIDE BY SELENIDE IN THE [4FE-4S] CLUSTERS OF THE FERREDOXIN FROM CLOSTRIDIUM-PASTEURIANUM

被引：26

作者：

MEYER, J ^{[1
]}

MOULIS, JM ^{[1
]}

机构：

[1] CEN, DEPT RECH FONDAMENTALE, BIOCHIM LAB, INSERM, U191, F-38041 GRENOBLE, FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1981年 / 103卷 / 02期

关键词：

D O I：

10.1016/0006-291X(81)90502-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The S atoms of the 2 [4Fe-4S] clusters present in the ferredoxin from C. pasteurianum were replaced by Se. The optical absorption spectrum of the Se-ferredoxin is slightly different from the spectrum of the native protein but it displays the characteristic features of [4Fe-4X] (X = S, Se) clusters. The reduced Se-ferredoxin can reduce hydrogenase and the oxidized Se-ferredoxin can be reduced by hydrogenase in the presence of H2. This is the 1st report of sulfide substitution by selenide in an Fe-S protein containing [4Fe-4S] active sites.

引用

页码：667 / 673

页数：7

共 21 条

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