SENSITIVE DETECTION AND EARLY PROGNOSTIC-SIGNIFICANCE OF P24 ANTIGEN IN HEAT-DENATURED PLASMA OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE 1-INFECTED INFANTS

被引：63

作者：

SCHUPBACH, J

BONI, J

TOMASIK, Z

JENDIS, J

SEGER, R

KIND, C

NADAL, D

WYLER, CA

CALAME, A

SCHAAD, UB

GNEHM, HP

KLINGLER, J

GIANINAZZI, MP

SCHUBIGER, G

BAUMGARTNER, C

KUCHLER, H

HUNZIKER, U

BUHLMANN, U

机构：

[1] UNIV ZURICH,CHILDRENS HOSP,DIV IMMUNOL & HEMATOL,ZURICH,SWITZERLAND

[2] KANTONSSPITAL,DIV NEONATOL,ST GALLEN,SWITZERLAND

[3] UNIV GENEVA,DEPT PEDIAT,CH-1211 GENEVA,SWITZERLAND

[4] UNIV LAUSANNE,DEPT PEDIAT,LAUSANNE,SWITZERLAND

[5] UNIV BASEL,DEPT PEDIAT,BASEL,SWITZERLAND

[6] CHILDRENS HOSP,AARAU,SWITZERLAND

[7] CHILDRENS HOSP,BIEL,SWITZERLAND

[8] CHILDRENS HOSP,LUGANO,SWITZERLAND

[9] CHILDRENS HOSP,LUZERN,SWITZERLAND

[10] CHILDRENS HOSP,ST GALLEN,SWITZERLAND

[11] CHILDRENS HOSP,SION,SWITZERLAND

[12] CHILDRENS HOSP,WINTERTHUR,SWITZERLAND

[13] STADTSPITAL TRIEMLI,CHILDRENS HOSP,ZURICH,SWITZERLAND

来源：

JOURNAL OF INFECTIOUS DISEASES | 1994年 / 170卷 / 02期

关键词：

D O I：

10.1093/infdis/170.2.318

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Immune complex formation causes underdetection of p24 antigen in human immunodeficiency virus (HIV) infection. Briefly boiling diluted plasma releases all complexed antigen, which can then be measured by some commercial assays. In a retrospective pediatric cohort study, the specificity of this procedure in 390 uninfected samples was 96.9% after initial testing and 100% after neutralization. Sensitivity among 125 postnatal infected samples was, at a detection of 2 pg/mL, 96.0% (97% neutralizable) compared with 47.7% for regular antigen (76% neutralizable), 96% for polymerase chain reaction, and 77% for viral culture. The high sensitivity and specificity of heat-denatured antigen was confirmed by prospectively testing 113 additional samples. Quantitative analysis of samples from infected infants showed low levels of p24 antigen in 29% of cord blood sera, a postnatal increase to levels that were during the first 6 months of life inversely associated with survival, and persistence of antigenemia thereafter independent of clinical status. Prevalence and antigen levels were significantly lower in mothers. The persistent antigenemia in children indicates that their immune systems cannot restrict HIV expression as efficiently as those of adults.

引用

页码：318 / 324

页数：7

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