CHANGE IN BINDING-PROPERTIES OF FOLATE-BINDING PROTEIN IN COWS WHEY DUE TO REMOVAL OF A COFACTOR DURING AFFINITY CHROMATOGRAPHIC PURIFICATION

被引：17

作者：

HANSEN, SI

HOLM, J

LYNGBYE, J

机构：

[1] Department of Clinical Chemistry, Research Division, Central Hospital

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 579卷 / 02期

关键词：

Affinity chromatography; Cofactor removal; Folate-binding protein; Milkwhey;

D O I：

10.1016/0005-2795(79)90077-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The folate-binding protein in cow's whey was purified by affinity chromatography on folate or methotrexate-AH-Sepharose® 4B. A change in basic binding properties of the protein occurred probably due to the fact that material removed during affinity chromatography contains a cofactor of great importance to folate binding. © 1979.

引用

页码：479 / 482

页数：4

共 7 条

[1] HANSEN SI, 1977, J CHROMATOGR, V134, P517, DOI 10.1016/S0021-9673(00)88553-2
[2] COOPERATIVE BINDING OF FOLATE TO A PROTEIN ISOLATED FROM COWS WHEY
HANSEN, SI
HOLM, J
LYNGBYE, J
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 535 (02) : 309 - 318
[3] HOLM J, 1978, ACTA PHARMACOL TOX, V42, P77
[4] Kaufman B T, 1974, Methods Enzymol, V34, P272
[5] ISOLATION OF FOLATE-BINDING PROTEIN FROM COWS MILK BY USE OF AFFINITY CHROMATOGRAPHY
SALTER, DN
SCOTT, KJ
FORD, JE
ANDREWS, P
[J]. FEBS LETTERS, 1972, 20 (03) : 302 - &
[6] CHEMICAL FIXATION OF FOLATE BINDING PROTEIN TO ACTIVATED SEPHAROSE
SELHUB, J
GROSSOWI.N
[J]. FEBS LETTERS, 1973, 35 (01) : 76 - 78
[7] PURIFICATION AND CHARACTERIZATION OF LOW-MOLECULAR WEIGHT HUMAN FOLATE BINDING-PROTEIN USING AFFINITY CHROMATOGRAPHY
WAXMAN, S
SCHREIBER, C
[J]. BIOCHEMISTRY, 1975, 14 (25) : 5422 - 5428

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