OLIGONUCLEOSIDE METHYLPHOSPHONATES AS ANTISENSE REAGENTS

被引：128

作者：

MILLER, PS

机构：

[1] Department of Biochemistry, School of Hygiene and Public Health, The Johns Hopkins University, Baltimore, MD

来源：

BIO-TECHNOLOGY | 1991年 / 9卷 / 04期

关键词：

D O I：

10.1038/nbt0491-358

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Oligonucleoside methylphosphonates contain nonionic internucleotide bonds that resist degradation by cellular nucleases and allow the oligomers to be taken up intact by mammalian cells in culture. Antisense methylphosphonate oligomers targeted against cellular or viral mRNA initation codon or coding regions or against precursor mRNA splice sites effectively and specifically inhibit mRNA expression in cells. The efficacy of antisense methylphosphonate oligomers can be enhanced by derivatization with functional groups that allow the oligomer to covalently cross-link with its targeted mRNA. These oligonucleotide analogs will be useful tools for studying and controlling gene expression and are also promising candidates for development as therapeutic agents.

引用

页码：358 / 362

页数：5

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