SELECTION FOR RESISTANCE TO FILTRATES OF FUSARIUM-SPP IN EMBRYOGENIC CELL-SUSPENSION CULTURE OF MEDICAGO-SATIVA L

被引：23

作者：

BINAROVA, P ^{[1
]}

NEDELNIK, J ^{[1
]}

FELLNER, M ^{[1
]}

NEDBALKOVA, B ^{[1
]}

机构：

[1] RES & BREEDING INST FORAGE CROPS, CS-66441 TROUBSKO, CZECHOSLOVAKIA

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 1990年 / 22卷 / 03期

关键词：

culture filtrate; disease resistance; embryogenic cell suspension culture; Fusarium spp; Medicago sativa L;

D O I：

10.1007/BF00033635

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A highly embryogenic cell suspension of alfalfa derived from a genotype sensitive to Fusarium oxysporum was successfully used for selection in vitro for resistance to culture filtrates of F. oxysporum, F. solani and F. avenaceum. Fifty two stable resistant cell lines were obtained and 500 plants regenerated from them. Among the 167 regenerants tested under glass there were 12-20% more plants with increased resistance to pathogens than in the group of plants regenerated from a control cell line. It was also found that the cell suspension cultures derived from genotypes of alfalfa with increased resistance to Fusarium spp. better tolerated filtrates of the pathogen. The results of a comparison of virulence of individual isolates of several species of Fusarium with toxicity of their filtrates to plants in vivo and in cell cultures were not unequivocal. © 1990 Kluwer Academic Publishers.

引用

页码：191 / 196

页数：6

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