PARTIAL MODIFICATION OF THE CARBAZOLE METHOD OF BITTER AND MUIR FOR QUANTITATION OF HEXURONIC ACIDS

The carbazole method of T. Bitter and H. M. Muir (1962, Anal. Biochem. 4, 330-334)for quantitation of hexuronic acids was modified partially. In this modification (procedure g), 0.2 m anhydrous sodium tetraborate was used instead of 0.025 m sodium tetraborate decahydrate. The color intensity of d-glucuronolactone was slightly diminished by this modification, however, that of l-iduronolactone significantly increased. Thus, the difference in color intensity between d-glucuronolactone and l-iduronolactone became very small. When evaluated by procedure g, the hexuronic acid content of dermatan sulfate was comparable to that of chondroitin sulfate A. © 1979 Academic Press, Inc.