USE OF A HPAII-POLYMERASE CHAIN-REACTION ASSAY TO STUDY DNA METHYLATION IN THE PGK-1 CPG ISLAND OF MOUSE EMBRYOS AT THE TIME OF X-CHROMOSOME INACTIVATION

被引：162

作者：

SINGERSAM, J

GRANT, M

LEBON, JM

OKUYAMA, K

CHAPMAN, V

MONK, M

RIGGS, AD

机构：

[1] UNIV LONDON UNIV COLL,MRC,MAMMALIAN DEV UNIT,LONDON NW1 2HE,ENGLAND

[2] NEW YORK STATE DEPT HLTH,ROSWELL PK MEM INST,DEPT MOLEC & CELLULAR BIOL,BUFFALO,NY 14263

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 09期

关键词：

D O I：

10.1128/MCB.10.9.4987

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A HpaII-PCK assay was used to study DNA methylation in individual mouse embryos. It was found that HpaII site H-7 in the CpG island of the X-chromosome-linked Pgk-1 gene is ≤10% methylated in oocytes and male embryos but becomes 40% methylated in female embryos at 6.5 days, about the time of X-chromosome inactivation of the inner cell mass.

引用

页码：4987 / 4989

页数：3

共 22 条

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