CYTOGENETIC EFFECTS OF VINCRISTINE SULFATE AND ETHYLENE DIBROMIDE IN HUMAN PERIPHERAL LYMPHOCYTES - MICRONUCLEUS ANALYSIS

被引:7
作者
CHANNARAYAPPA
ONG, T
NATH, J
机构
[1] W VIRGINIA UNIV,COLL AGR & FORESTRY,DIV PLANT & SOIL SCI,MORGANTOWN,WV 26506
[2] NIOSH,DIV RESP DIS STUDIES,MORGANTOWN,WV 26505
关键词
HUMAN LYMPHOCYTES; MICRONUCLEUS ASSAY; MONONUCLEATED AND BINUCLEATED CELLS; CYTOKINESIS-BLOCK;
D O I
10.1002/em.2850200207
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Micronuclei kinetics and persistence in mononucleated and binucleated human peripheral lymphocytes following short-term (4 hr) and continuous (until harvest) in vitro exposure to vincristine sulfate (VS) and ethylene dibromide (EDB) were studied. Lymphocytes were exposed to chemicals for various doses and harvested at different culture times. Micronucleus frequencies were scored in both mononucleated and binucleated cells on the same slide. VS-treated cells showed a significantly higher incidence of micronucleus in both mononucleated and binucleated cells than controls (P < 0.01). The cells treated continuously with VS produced comparatively higher frequencies of micronucleated cells than those treated for 4 hr. Highest micronuclei frequencies were observed 24 hr after chemical treatment in both mononucleated and binucleated cells and decreased later with time. However, the micronucleus frequencies remained significantly higher than the controls even in the cells harvested at 144 hr. VS induced a large number of micronucleated cells with multiple micronuclei. VS also caused a severe decrease in nuclear division due to cytotoxic effect. Lymphocytes treated with EDB for 4 hr and continuously showed a statistically higher incidence of micronuclei in binucleated cells compared to the controls (P < 0.05), whereas in mononucleated cells higher micronucleus frequencies were observed only in cultures treated continuously. Continuous presence of EDB induced both dose- and time-dependent increase of micronuclei in both mono- and binucleated cells (P < 0.05). EDB induced relatively few multiple micronucleated cells in comparison with VS. EDB did not affect nuclear divisions even with continuous treatment. High micronucleus frequencies observed at 144 hr harvest following 4 hr treatment of both EDB and VS suggest the persistence of DNA damage in cells. These studies suggest that micronuclei kinetics in human peripheral lymphocytes depends on the genotoxic potentially and cytotoxicity of a genotoxicant.
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页码:117 / 126
页数:10
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