FREE BETA-CHORIONIC GONADOTROPIN - A CROSS-REACTIVITY STUDY OF 2 IMMUNOMETRIC ASSAYS USED IN PRENATAL MATERNAL SERUM SCREENING FOR DOWNS-SYNDROME

被引：24

作者：

MACRI, JN ^{[1
]}

SPENCER, K ^{[1
]}

ANDERSON, RW ^{[1
]}

COOK, EJ ^{[1
]}

机构：

[1] OLDCHURCH HOSP,DEPT CLIN BIOCHEM,ENDOCRINE UNIT,ROMFORD RM7 0BE,ESSEX,ENGLAND

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1993年 / 30卷

关键词：

AFFINITY PURIFICATION; ASSAY VALIDATION; GLYCOPROTEIN HORMONES;

D O I：

10.1177/000456329303000117

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

We have evaluated the cross-reactivity characteristics of two distinct immunometric assays for the measurement of free beta-human chorionic gonadotropin (free beta). These maternal serum assays have been used in the initial studies which evaluated free beta as a marker in the prenatal detection of Down's syndrome. It has been suggested that free beta assays are subject to substantial potential for cross-reactivity. To confirm that free beta was the analyte responsible for enhanced detection efficiency both non-competitive and competitive cross-reactivity evaluations were undertaken. These studies demonstrated acceptably small cross-reactivity to other glycoprotein hormones or their beta components. We conclude that properly designed free beta assays will provide specific analyte measurement and improved detection efficiency in Down's syndrome screening.

引用

页码：94 / 98

页数：5

共 5 条

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[5]

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