DETECTION OF NON-CULTURABLE VIBRIO-CHOLERAE O139, BY PCR AND FLUORESCENT-ANTIBODY METHODS, IN LABORATORY MICROCOSMS

被引：4

作者：

ISLAM, MS

ALAM, MJ

MIAH, MA

FELSENSTEIN, A

SACK, RB

机构：

[1] Environmental Microbiology Laboratory, Laboratory Sciences Division, International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR, B), Dhaka, 1000

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1995年 / 11卷 / 05期

关键词：

MICROCOSM; NON-CULTURABLE; PCR; VIBRIO CHOLERAE O139;

D O I：

10.1007/BF00286382

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Non-culturable Vibrio cholerae O139 was detected in microcosms by PCR and fluorescent-antibody (FA) techniques. When survival of V. cholerae O139 in microcosms was assessed by viable counting on culture media, the vibrio became non-culturable after 44 days and remained non-culturable for an additional 7 weeks.

引用

页码：597 / 598

页数：2

共 6 条

[1] VIABLE BUT NON-CULTURABLE VIBRIO-CHOLERAE AND RELATED PATHOGENS IN THE ENVIRONMENT - IMPLICATIONS FOR RELEASE OF GENETICALLY ENGINEERED MICROORGANISMS [J].

COLWELL, RR ;

BRAYTON, PR ;

GRIMES, DJ ;

ROSZAK, DB ;

HUQ, SA ;

PALMER, LM .

BIO-TECHNOLOGY, 1985, 3 (09) :817-820

[2]

COLWELL RR, 1990, ADV RES CHOLERA RELA, P327

[3] CHOLERA-TOXIN GENE POLYMERASE CHAIN-REACTION FOR DETECTION OF NON-CULTURABLE VIBRIO-CHOLERAE O1 [J].

HASAN, JAK ;

CHOWDHURY, MAR ;

SHAHABUDDIN, M ;

HUQ, A ;

LOOMIS, L ;

COLWELL, RR .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1994, 10 (05) :568-571

[4] ISOLATION OF VIBRIO-CHOLERAE O139 SYNONYM BENGAL FROM THE AQUATIC ENVIRONMENT IN BANGLADESH - IMPLICATIONS FOR DISEASE TRANSMISSION [J].

ISLAM, MS ;

HASAN, MK ;

MIAH, MA ;

YUNUS, M ;

ZAMAN, K ;

ALBERT, MJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (05) :1684-1686

[5] SEASONALITY AND TOXIGENICITY OF VIBRIO-CHOLERAE NON-01 ISOLATED FROM DIFFERENT COMPONENTS OF POND ECOSYSTEMS OF DHAKA-CITY, BANGLADESH [J].

ISLAM, MS ;

ALAM, MJ ;

NEOGI, PKB .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1992, 8 (02) :160-163

[6] POLYMERASE CHAIN-REACTION FOR DETECTION OF THE CHOLERA ENTEROTOXIN OPERON OF VIBRIO-CHOLERAE [J].

SHIRAI, H ;

NISHIBUCHI, M ;

RAMAMURTHY, T ;

BHATTACHARYA, SK ;

PAL, SC ;

TAKEDA, Y .

JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (11) :2517-2521

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