HIGH EXPRESSION OF BACILLUS-LICHENIFORMIS ALPHA-AMYLASE WITH A BACILLUS SECRETION VECTOR

被引：16

作者：

SIBAKOV, M ^{[1
]}

机构：

[1] UNIV HELSINKI, RECOMBINANT DNA LAB, SF-00380 HELSINKI, FINLAND

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1986年 / 155卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1986.tb09527.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gene coding for the heat-stable .alpha.-amylase from Bacillus licheniformis ATCC 14580 has been expressed with the aid of a B. amyloliquefaciens .alpha.-amylase-based expression/secretion vector by joining the structural part of the gene to a pool of vectors after the B. amyloliquefaciens .alpha.-amylase promoter and signal sequence. The recombinant plasmids obtained were stably maintained in B. subtilis and the heat-stable .alpha.-amylase activity rose several hundred times from the level of the donor. Eight different constructions were further analyzed. Each of them had an intact B. amyloliquefaciens signal sequence, the only difference being in a few nucleotides beyond the C terminus of the signal peptide. This, however, was enough to cause up to fourfold differences in protein yield. Possible reasons for the variation in the production level are discussed.

引用

页码：577 / 581

页数：5

共 24 条

[1] CLONING AND EXPRESSION OF THERMOSTABLE ALPHA-AMYLASE GENE FROM BACILLUS-STEAROTHERMOPHILUS IN BACILLUS-STEAROTHERMOPHILUS AND BACILLUS-SUBTILIS [J].

AIBA, S ;

KITAI, K ;

IMANAKA, T .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1983, 46 (05) :1059-1065

[2]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[3] SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN ESCHERICHIA COLI - PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM [J].

CLEWELL, DB ;

HELINSKI, DR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1969, 62 (04) :1159-&

[4] CLONING AND EXPRESSION OF A BACILLUS-COAGULANS AMYLASE GENE IN ESCHERICHIA-COLI [J].

CORNELIS, P ;

DIGNEFFE, C ;

WILLEMOT, K .

MOLECULAR AND GENERAL GENETICS, 1982, 186 (04) :507-511

[5]

GROSCH JC, 1983, AM SOC MICROBIOL ANN

[6] MOLECULAR-CLONING OF HETEROLOGOUS CHROMOSOMAL DNA BY RECOMBINATION BETWEEN A PLASMID VECTOR AND A HOMOLOGOUS RESIDENT PLASMID IN BACILLUS-SUBTILIS [J].

GRYCZAN, T ;

CONTENTE, S ;

DUBNAU, D .

MOLECULAR & GENERAL GENETICS, 1980, 177 (03) :459-467

[7] CLONING OF A THERMOSTABLE ALPHA-AMYLASE GENE FROM BACILLUS-LICHENIFORMIS AND ITS EXPRESSION IN ESCHERICHIA-COLI AND BACILLUS-SUBTILIS [J].

JOYET, P ;

GUERINEAU, M ;

HESLOT, H .

FEMS MICROBIOLOGY LETTERS, 1984, 21 (03) :353-358

[8] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[9]

Maxam A M, 1980, Methods Enzymol, V65, P499

[10] RUBELLA-VIRUS CONTAINS ONE CAPSID PROTEIN AND 3 ENVELOPE GLYCOPROTEINS, E1, E2A, AND E2B [J].

OKERBLOM, C ;

KALKKINEN, N ;

KAARIAINEN, L ;

PETTERSSON, RF .

JOURNAL OF VIROLOGY, 1983, 46 (03) :964-973

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